mouse brain cdna library (OriGene)
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mouse brain cdna library
Mouse Brain Cdna Library, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+brain+cdna+library/SOX10+(BC002824)+Human+Untagged+Clone/pmc02632752-179-30-40
Average 90 stars, based on 3 article reviews
Mouse Brain Cdna Library, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+brain+cdna+library/SOX10+(BC002824)+Human+Untagged+Clone/pmc02632752-179-30-40
Average 90 stars, based on 3 article reviews
mouse brain cdna library - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "At Least Ten Genes Define the Imprinted Dlk1-Dio3 Cluster on Mouse Chromosome 12qF1"
Article Title: At Least Ten Genes Define the Imprinted Dlk1-Dio3 Cluster on Mouse Chromosome 12qF1
Journal: PLoS ONE
doi: 10.1371/journal.pone.0004352
Figure Legend Snippet: This figure summarizes our results about the imprinting and structure of genes in this mouse region. Arrows denote transcriptional orientations. Genes written in black were shown to be biallelically expressed. Genes drawn in blue are paternally expressed, while those in red are maternally expressed. Genes written in yellow were undetectable by RT-PCR. For Begain transcribed from promoter 2, this transcript was coded blue as it is paternally expressed in sheep in a tissue specific manner, although in this study, RT-PCR failed to amplify a transcript derived from this promoter. Of these, accession numbers, AK141557, AK163826, AK048151, and AK044800, correspond to unspliced “transcripts” whose cDNA sequence ends in a polyA stretch of genomic DNA, suggesting that these “cDNAs” may be genomic DNA contamination in the Riken mouse cDNA libraries. For the bottom panel, exons are dark colored while introns are light. Purple lines represent the position of microRNA precursors. In all, fifty-two mouse microRNA precursors as listed in map to this imprinted region.
Techniques Used: Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Sequencing
Figure Legend Snippet: (A) A DraIII restriction site polymorphism between 129S1 and CzechII/Ei mice was utilized to determine that Dlk1 is paternally expressed. PCR primers were Dlk1 2Up/317Dn. (B) Northern analysis using the same Dlk1 PCR fragment as probe revealed that Dlk1 is widely expressed. Of the tissues investigated, only the adult brain is characterized by having an additional and abundant transcript that is roughly 4.5 kb in length. (C) Several ESTs cluster to the syntenic region of “DAT” ( Dlk1 associated transcript). RT-PCR was done with “DAT” primers 1533Up/2044Dn. Using a EcoO109I restriction site polymorphism, “DAT” was shown to be like Dlk1 in its paternal expression. (D) Northern analysis on adult tissue samples of “DAT” detected a 4.5 kb transcript only in the brain. This band is identical in size to the long transcript of Dlk1 found in the brain. Moreover, the length of this transcript is greater than the distance (∼2.7 kb) between mouse Dlk1 polyA and “DAT” polyA. (E) RT-PCR was performed with various primer sets. Left most panel showed that Dlk1 is expressed in brain, heart and muscle. Middle panel shows that “DAT” is only detectable in brain and is consistent with Northern findings. For the right panel, PCR was performed with a primer set in which the upstream primer ( Dlk1 1237Up) was before the canonical Dlk1 polyA site while the downstream primer was after this site ( Dlk1 1805Dn). A band was detected only in the brain, indicating that “DAT” is an alternatively polyadenylated transcript of Dlk1 . This result was confirmed by cDNA cloning (Accession numbers: EU434914-EU434917).
Techniques Used: Northern Blot, Reverse Transcription Polymerase Chain Reaction, Expressing, Clone Assay
Figure Legend Snippet: Mouse cDNAs Cloned in This Study.
Techniques Used: Clone Assay, Northern Blot
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